Caspase Activity Assays

A distinctive feature of the early stages of apoptosis is the activation of caspase enzymes, which participate in the cleavage of protein substrates and in the subsequent disassembly of the cell. We offer a series of caspase assays that allow the simple detection of active caspases in living cells in real time or as a snapshot.

• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Simple work-flow
• High throughput
 

Product Name	Cat. #	Price	View
Caspase-3/7 Apoptosis Assay Kit (100 rxns)	C0046	$249

Mitochondrial Membrane Potential Assays

JC1 Mitochondrial Membrane Potential Detection Kit provides a quick and reliable method to detect the mitochondrial membrane potential changes in apoptotic cells using flow cytometry.

• Simple: add, incubate, and read format, no wash needed.
• Flexibility: suitable for flow cytometry, fluorescence microscope, and plate reader.
• Fast: results in as little as 30 minutes.
 

Product Name	Cat. #	Price	View
JC1 Mitochondrial Membrane Potential Detection Kit (100 rxns)	C0047	$199

Case Study

Detection of apoptosis activity in Jurkat cells using the Caspase-3/7 Apoptosis Assay Kit‎.

Detection of cell apoptosis with JC1 Mitochondrial Membrane Potential Detection Kit. Jurkat cells treated with 10 μM camptothecin for 4 hr (right panel) or untreated (left panel). Cells were stained with JC-1 reagent, then analyzed by flow cytometry.