In the TUNEL assays, the DNA strand breaks with 3'-hydroxyl ends are labeled with biotin-11-dUTP in the presence of terminal deoxynucleotidyl transferase (TdT). Once incorporated into the DNA, Biotin is detected using HRP or fluorophore labeled streptavidin. Our kits provide sufficient reagents for 50 assays, and are suitable for cell and tissue samples.
 

Key Features

• Broad application: Suitable for FFPE tissue, frozen tissue and cells.

• High sensitivity: Can detect a low population of apoptotic cells.

• Multicolor: Many fluorophores to choose.
 

Product Name	Cat. #	Price	View
TUNEL Chromogenic Apoptosis Detection Kit (50 rxns)	C0036	$439
TUNEL LiFluor™ 488 Apoptosis Detection Kit (50 rxns)	C0037	$439
TUNEL LiFluor™ 594 Apoptosis Detection Kit (50 rxns)	C0038	$169
TUNEL LiFluor™ 647 Apoptosis Detection Kit (50 rxns)	C0039	$189

Case Study

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue. The imaging results using TUNEL LiFluor™ 488 Apoptosis Detection Kit (left), and TUNEL Chromogenic Apoptosis Detection Kit (right).