Cell Transfection Reagents
Based on our unique polymer synthesis technologies, currently we are developing and manufacturing four categories of transfection reagents, which show distinct transfection characteristics compared with the leading products in the market. What's more important is that the transfection reagents we are manufacturing are very affordable and will save your precious research budget.
Selection Guide
| Reagents | Features | Sample & Cell Type | Replacement |
| PeneFect™ | • Excellent for both single DNA transfection and multi DNA co-transfection • Simple & robust transfection procedure • Cost-effective |
DNA Mammalian cells |
Fugene HD |
| LiFect293™ | • Best for suspension 293 cells • High levels of recombinant protein production • Presence of serum and antibiotics enhances efficiency on 293 cells • Exceptional efficiency for both single DNA transfection and multi DNAs co-transfection |
DNA HEK293 related cells |
ExpiFectamine 293 |
| LiFectRNA™ | • Simple and rapid protocol • Superior transfection efficiency • Compatible with a broad range of cell types • Low cytotoxicity |
siRNA or miRNA | Lipofectamine RNAiMAX |
| LiFect2K™ | • Superior transfection efficiency for a broad range of cell lines • Does not require removal of serum or culture medium • Does not require washing or changing of medium after transfection • Low cytotoxicity |
DNA, RNA BHK-21, K562, NIH 3T3 |
LipoFect2000 |
| LiFect2K™ | • Superior transfection efficiency for difficult-to-transfect cells • Does not require removal of serum or culture medium • Does not require washing or changing of medium after transfection • Improved cell viability: Gentle on your cells with low toxicity |
DNA, RNA & DNA/RNA Hard-to-transfect cells |
LipoFect3000 |
| Product Name | Cat. # | Price | View |
| PeneFect™ Transfection Reagent (1 ml) | M0001-01 | $199 |  |
| LiFect293™ Transfection Reagent (1 ml) | M0002-01 | $249 | |
| LiFectRNA™ Transfection Reagent (1 ml) | M0146-01 | $279 | |
| LiFect2K™ Transfection Reagent (1 ml) | M0003-01 | $229 | |
| LiFect3K™ Transfection Reagent (0.75 ml) | M0004-01 | $329 | |
Case Study
Figure A & B. Assessment of LiFect2KTM transfection efficiencies for siRNA. Hela cell line was transfected with either a GFP cDNA (A), or co-tranfected with GFP cDNA and an anti-GFP siRNA (B). Transfection efficiencies were assessed by GFP signal.
Figure C & D. Comparison of LiFect2KTM and Lipofectamine 2000 in plasmid transfection on mouse neuronal cells. Mouse neuronal cells were transfected with a GFP-expressing plasmid using either LiFect2K™ transfection reagent (C), or Lipofectamine 2000 (D). Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).
Assessment of LiFect3K™ transfection efficiencies using GFP visualization. A549, NIH3T3, HepG2, and MCF7 cell lines were transfected with a GFP-expressing plasmid using the indicated transfection reagents. Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).
Publications
1. Intranasal pediatric parainfluenza virus-vectored SARS-CoV-2 vaccine is protective in monkeys.
Publication: Cell Product: LiFect293™ Transfection Reagent
2. Increased neutralization potency and breadth elicited by a SARS-CoV-2 mRNA vaccine forming virus-like particles.
Publication: PNAS Product: LiFect293™ Transfection Reagent
3. A multiclade env–gag VLP mRNA vaccine elicits tier-2 HIV-1-neutralizing antibodies and reduces the risk of heterologous SHIV infection in macaques.
Publication: Nature Medicine Product: LiFect293™ Transfection Reagent
4. Live-attenuated pediatric parainfluenza vaccine expressing 6P-stabilized SARS-CoV-2 spike protein is protective against SARS-CoV-2 variants in hamsters.
Publication: PLOS Product: LiFect293™ Transfection Reagent
5. Functional Anatomy of the Trimer Apex Reveals Key Hydrophobic Constraints That Maintain the HIV-1 Envelope Spike in a Closed State.
Publication: ASM Product: LiFect293™ Transfection Reagent
