DNA libraries refer to a collection of large numbers of DNA sequences that have been cloned into vectors. Life scientists working in fields such as protein engineering, antibody engineering, enzyme engineering, synthetic biology, discovery biology, and structural biology can identify and isolate the DNA fragments interested for further study. LifeSct has many years of experience in gene synthesis and can provide customers with a variety of custom DNA library services.
 

Site-directed Mutagenesis Library

Site-directed mutagenesis library involves sequentially substituting a customer-determined amino acid position with all 19 other amino acids to generate a mutant library. This method enables scientists to conduct structural-functional analysis, examining the impacts of specific amino acid substitutions, both beneficial and adverse.

Capacity

Individual, 100% sequence-verified clones

Delivery Forms

1. 2-5 µg of lyophilized plasmid DNA
2. Restriction digest map
3. Sequence trace data with alignment
4. Sequence files of the synthetic gene alone or subcloned into a vector
 

Scanning Library

Scanning Library involves sequentially substituting specific residues within a customer-defined region with one specified residue via site-directed mutagenesis. Alanine and Cysteine scanning are two commonly utilized types of scanning libraries.

Capacity

Individual, 100% sequence-verified clones

Delivery Forms

1. 2-5 µg of lyophilized plasmid DNA
2. Restriction digest map
3. Sequence trace data with alignment
4. Sequence files of the synthetic gene alone or subcloned into a vector
 

Truncation Library

A truncation library of mutants is constructed by trimming amino acids from either the N- or C-terminus (or both) of a protein around a specified core region. This enables scientists to pinpoint the core region responsible for protein function.

Capacity

Individual, 100% sequence-verified clones

Delivery Forms

1. 2-5 µg of lyophilized plasmid DNA
2. Restriction digest map
3. Sequence trace data with alignment
4. Sequence files of the synthetic gene alone or subcloned into a vector
 

Randomized Library

A randomized mutant library enables random amino acid substitutions within a user-defined region (e.g., ORF region within 200bp-1500bp). These proteins can be expressed and assayed for function to identify beneficial substitutions in protein engineering studies.

Capacity

Ready-to-clone PCR Fragment Library or Cloned Pooled Library Up to 109 variants.

Delivery Forms

1. PCR fragment Library: 2-4 µg of linear ds DNA (ready-to-clone via 5’ and 3’ restriction sites if desired)
2. Cloned pool Library: 5-10 µg of lyophilized plasmid DNA and Glycerol stock of total library with up to 109 transformants
3. Sequence verification of a pre-determined number of clones (with statistical analysis) based on library size
 

Degenerated Library

Life Scientists can create a library of mutants by combining various genetic elements in multiple combinations. LifeSct provides both traditional partially randomized (NNK/NNS) and completely randomized (NNN) mutant library services to support this endeavor.

Capacity

Ready-to-clone PCR Fragment Library or Cloned Pooled Library Up to 109 variants.

Delivery Forms

1. PCR fragment Library: 2-4 µg of linear ds DNA (ready-to-clone via 5’ and 3’ restriction sites if desired)
2. Cloned pool Library: 5-10 µg of lyophilized plasmid DNA & Glycerol stock of total library with up to 109 transformants
3. Sequence verification of a pre-determined number of clones (with statistical analysis) based on library size