Labeled Nucleotides
LIFE SCB offers 5′-aminoallyl dUTP and UTP analogs, as well as conjugates with biotin and various fluorescent dyes, featuring our exceptionally bright and photostable LiFluor dyes. Fluorescent and biotin-labeled dUTP and UTP are widely used for enzymatic incorporation into DNA or RNA.
Key Features
• Bright signals with low background.
• Wide variety of fluorophores.
• Great for many applications, like Fluorescence in situ Hybridization (FISH), TUNEL, and more.
| Product Name | Cat. # | Price | View |
| 5-Aminoallyl-dUTP (100 μl) | M0070 | $149 |  |
| 5-Aminoallyl-UTP (100 μl) | M0071 | $149 | |
| Biotin-11-dUTP (50 μl) | M0072 | $199 | |
| Biotin-11-UTP (50 μl) | M0073 | $199 | |
| Biotin-14-dCTP (50 nmol) | M0074 | $389 | |
| Biotin-14-dATP (50 nmol) | M0044 | $399 | |
| 5-FAM-dUTP (25 μl) | M0075 | $169 | |
| 5-FAM-X-dUTP (25 μl) | M0076 | $199 | |
| 5-FAM-UTP (25 μl) | M0077 | $169 | |
| 5-FAM-X-UTP (25 μl) | M0078 | $199 | |
| LiFluor™ 488-X-dUTP (25 μl) | M0079 | $299 | |
| LiFluor™ 488-X-UTP (25 μl) | M0080 | $299 | |
| LiFluor™ 555-X-dUTP (25 μl) | M0081 | $299 | |
| LiFluor™ 555-X-UTP (25 μl) | M0082 | $299 | |
| LiFluor™ 568-X-dUTP (25 μl) | M0083 | $299 | |
| LiFluor™ 568-X-UTP (25 μl) | M0084 | $299 | |
| LiFluor™ 594-X-dUTP (25 μl) | M0085 | $299 | |
| LiFluor™ 594-X-UTP (25 μl) | M0086 | $299 | |
| LiFluor™ 647-X-dUTP (25 μl) | M0087 | $299 | |
| LiFluor™ 647-X-UTP (25 μl) | M0088 | $299 | |
| Cy3-X-dUTP (25 μl) | M0142 | $259 | |
| Cy3-X-UTP (25 μl) | M0143 | $259 | |
| Cy5-X-dUTP (25 μl) | M0144 | $259 | |
| Cy5-X-UTP (25 μl) | M0145 | $259 | |
Case Study
Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with LiFluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with LiFluor 488-X-dUTP (green) and LiFluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.
Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with LiFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).
