AAV Packaging
Adeno-associated virus (AAV), part of the parvovirus family, features a single-stranded DNA genome of approximately 4.8 kb and lacks an envelope. Infectious AAV can only be produced in the host with the assistance of helper viruses such as adenovirus or herpes virus. Experience LIFESCT's superior AAV packaging service. Using plasmid recombination systems and advanced density gradient centrifugation, we perfectly concentrate and purify the virus. Our strict quality control ensures titers up to 10^13 vg/ml, meeting the needs of cell infection and in vivo experiments.
Key Features
• Nonpathogenic with least immune response.
• Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
• Persistent transgene expression.
• Multiple serotypes (AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-7, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ).
Selection Guide
| Scale | Package | Turnaround time | Price |
|
Medium |
Customized Over-expression AAV (gene ≤ 2.8 kb), Deliverables: ≥ 1012 vg/ml, 1 ml |
25 business days | Quote |
| RNAi/sgRNA AAV(sgRNA only, no Cas9 gene), Deliverables: ≥ 1012 vg/ml, 1 ml |
25 business days | Quote | |
| miRNA-up/down-AAV, Deliverables: ≥ 1012 vg/ml, 1 ml |
25 business days | Quote | |
|
|
Customized Over-expression AAV (Gene ≤ 2.8kb), Deliverables: ≥ 1013 vg/ml, 1 ml |
25 business days | Quote |
| RNAi/sgRNA AAV (sgRNA only , no Cas9 gene), Deliverables: ≥ 1013 vg/ml, 1 ml |
25 business days | Quote |
Deliverables
| Custom AAV, delivered to specification: Medium Scale: ≥ 1012 vg/ml, 5× 200μl Large Scale: ≥ 1013 vg/ml, 5× 200μl |
| Free: Control virus Medium Scale: ≥ 1012vg/ml, 5× 200μl Large Scale: ≥ 1013vg/ml, 5× 200μl |
| Dry ice/-80 ° C, avoid repeated freezing and thawing |
