mRNA Synthesis
High-quality mRNA serves as a crucial tool in various scientific, medical, and biotechnological applications, especially in the mRNA vaccine and therapy development. The reliability and purity of the mRNA are essential for achieving accurate and reproducible results. In vitro transcribed (IVT) mRNA synthesis lies in its ability to provide researchers and life scientists with a customizable, rapid, and controlled method for generating high-quality mRNA molecules for diverse applications. LIFE SCB specializes in providing cutting-edge IVT mRNA synthesis services. Our state-of-the-art facilities and experienced team ensure the production of high-quality synthetic mRNA tailored to meet your specific research or discovery needs.
Key Features
• One-stop solution: from codon optimization, plasmid preparation, to in vitro transcription of mRNA and purification.
• Flexible synthesis specifications: a variety of modifications, capping, quantity, purity are available.
• Strict quality control: standard QC items and a variety of customized QC items can be provided according to customer requirements.
Specifications
| Process | Description | Deliverables | Turnaround Time |
| 1. Gene synthesis | Codon optimization, Gene synthesis and cloning into vector | Sanger sequencing report | 1-2 weeks |
| 2. Plasmid Preparation | Plasmid preparation and linearization | Lyophilized plasmid | 1 week |
| 3. IVT mRNA | In Vitro Transcription | Lyophilized or liquid mRNA | Quote |
| 4. Standard QC items | Appearance, mRNA length, sequence accuracy, RNA concentration, A260/280, purity | COA report | Quote |
| 5. Customized QC items (optional) | PolyA tailing efficiency, capping efficiency, template DNA residue, protein residue, RNase residue, dsRNA detection, endotoxin content, bioburden. | COA report | Quote |
