DNA-seq
Human whole genome sequencing enables researchers to catalog the genetic constitution of individuals and capture all the variants present in a single assay. It is applied to the study of cancer and a variety of diseases, as well as human population evolution studies and pharmacogenomics.
Key Features
1. State-of-the-art NGS technologies: LifeSct is a world leader in sequencing capacity using state-of-the-art technology, including the latest generation Illumina HiSeq X Ten.
2. Highest data quality: We guarantee a Q30 score ≥ 80%, exceeding Illumina’s official guarantee of ≥75%.
3. Extraordinary informatics expertise: LifeSct uses its cutting-edge bioinformatics pipeline and internationally recognized best-in-class software to provide customers highly reliable “publication-ready data”.
Service Options (Cat. #: S0026)
| Project | Description | Unit Price | Turnaround Time |
|
|
Library Preparation | $150/sample |
15-20 business days |
| Sequencing | $25/G | ||
| FTP | $50 |
Service Overview
| Sequencing Strategy: | 1. 350 bp insert DNA library 2. HiSeq X platform, paired-end 150 bp |
| Data Quality Guarantee: | We guarantee that ≥ 80% of bases have a sequencing quality score ≥ Q30, which exceeds Illumina’s official guarantee of ≥ 75%. |
| Sample Requirements: | 1. Input DNA: For fresh sample: ≥ 1.0 μg (a minimum of 200 ng can be accepted with risk); For FFPE sample: ≥ 1.5 μg 2. DNA concentration: ≥ 20 ng/μl 3. DNA volume: ≥ 10 μl 4. Purity: OD260/280 = 1.8 - 2.0 without degradation or RNA contamination |
| Turnaround Time: | 1. 15 working days after verification of sample quality (without data analysis) 2. Additional 8 working days for data analysis |
| Recommended Sequencing Depth: | 1. For tumor tissues: 50×, adjacent normal tissues and blood 30× 2. For rare diseases: 30~50× |
| Bioinformatics Analysis: | 1. Data quality control: filtering out reads containing adapters or with low quality 2. Alignment with reference genome, statistics of sequencing depth and coverage 3. SNP/InDel/SV/CNV calling, annotation and statistics 4. Somatic SNP/InDel/SV/CNV calling, annotation and statistics (paired tumor samples) |
