LiGreen™ Plus Nucleic Acid Gel Stain is an exceptionally sensitive fluorescent stain designed for the detection of nucleic acids within agarose and polyacrylamide gels. This versatile stain offers high-sensitivity detection for both double-stranded and single-stranded DNA and RNA. It provides flexibility in application, allowing for post-staining of gels or incorporation into agarose gels during the casting process. LiGreen™ Plus is designed to be compatible with various light sources, including a standard 300 nm transilluminator, a 254 nm transilluminator, a blue-light transilluminator, as well as gel readers equipped with visible light excitation such as a 488 nm laser-based gel scanner, ensuring broad applicability and ease of use in various laboratory settings.
Gel staining with LiGreen™ Plus is compatible with downstream applications such as gel extraction and cloning. LiGreen™ Plus is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.
Key Features
• Safer than EB. Shown by Ames test and other tests to be non-mutagentic and non-cytotoxic.
• Stable at room temperature.
• Compatible with standard blue LED and UV transilluminators.
• Use with blue light reduces mutagenesis for gel excision and subsequent cloning.
Component
LiGreen™ Plus Nucleic Acid Gel Stain (10,000× in DMSO): 500 μl
Storage
Store at 2-25°C and protect from light.
Case Study
Gel stain results with LiGreen™, LiGreen™ Plus, and LiGreen™ Ultra (Left), and comparison results with SYBR Safe and GelGreen (Right). Left: Two-fold serial dilutions of a 1 kb DNA ladder were loaded onto each gel in 3 lanes in the amounts of 25 ng, 50 ng and 100 ng (left to right), then post-stained with LiGreen™, LiGreen™ Plus, and LiGreen™ Ultra. Right: Results show LiGreen™ has similar sensitivity with SYBR Safe and GelGreen™, LiGreen™ Plus and LiGreen™ Ultra have much better sensitivity.
Cell permeability tests with SYBR Safe, LiGreen™, LiGreen™ Plus, and LiGreen™ Red. Hela cells were incubated at 37ºC with 1× SYBR Safe, LiGreen™, LiGreen™ Plus, and LiGreen™ Red for 30 min. LiGreen™ Plus and LiGreen™ Red could not penetrate cell membranes to bind DNA in living cells, and were much safer than SYBR Safe, which rapidly entered cells and stained nuclei.