PeneFect™ DNA Transfection Reagent for HEK293, CHO & Hard-to-Transfect Cells
PeneFect™ Transfection Reagent is a high-performance, biodegradable polymer-based solution engineered for superior DNA delivery. Developed using proprietary synthesis technology, PeneFect™ provides effective, reproducible transfection across common cell lines and hard-to-transfect mammalian cells, including HEK293, CHO, HeLa, COS-7, and NIH-3T3.
Why Choose PeneFect™ Reagent for Your Lab?
1. Our unique biodegradable formula degrades rapidly following endocytosis, ensuring high cell viability.
2. Form stable transfection complexes in just 5 minutes at room temperature.
3. Optimized for both standard recombinant protein production and large DNA constructs (>89 kb).
4. 1.0 ml provides sufficient reagent for ~667 transfections (24-well) or ~333 transfections (6-well), making it a budget-friendly alternative to market-leading brands.
Key Features & Benefits
1. Biodegradable Technology: Fully degrades post-delivery to protect delicate cell phenotypes.
2. Superior Viral Titers: Specifically optimized for high-yield virus production.
3. Complex Co-Transfections: Exceptional stability for both single DNA and multi-DNA co-transfection protocols.
4. Broad Compatibility: Reliable results on a wide range of challenging mammalian cell lines.
5. Simplified Protocol: A robust, user-friendly procedure designed for high-throughput consistency.
Components
1. PeneFect™ Transfection Reagent: 1 ml
Storage
Store at 4°C.
Other Size
| Product Name | Cat. # | Price | View |
| PeneFect™ Transfection Reagent (4 ml) | M0001-04 | $529 |  |
Case Study
A comparative analysis of transfection efficiency between PeneFect™ reagent and Lipofectamine 2000, was performed on HEK-293T cells.
HEK-293T cells were transfected with a GFP expression vector (pEGFP-N3) using PeneFect™ (left panel) or Lipofectamine 2000 (right panel). Transgene expression was assessed 24 hours post-transfection using a Nikon Eclipse fluorescence microscope.
A comparative analysis of transfection efficiency between PeneFect™ reagent and Lipofectamine 2000
, was performed on HepG2 cells. HepG2 cells were transfected with a GFP expression vector (pEGFP-N3) using PeneFect™ (left panel) or Lipofectamine 2000 (right panel). GFP expression was visualized 24 hours post-transfection using a Nikon Eclipse fluorescence microscope.