IntactProtein™ Cell-Tissue Lysis Kit includes a uniquely formulated protein lysis and extraction buffer mix. This innovative and proprietary reagent mix will greatly facilitate researchers to extract proteins of all sizes from both cultured mammalian tissues and cells.
 

Key Features

• All-in-one formula: No protease/PTM inhibitors needed; no sonication required.

• Ready-to-use protocol: Simply mix Reagents A&B; extraction in as little as 15 min.

• Ultimate solution for large proteins: Near-complete extraction; no fragmentation.

• Assurance and peace of mind: No loss of protein PTMs such as phosphorylation.

• All-around performance: Suitable for mammalian cells and tissues.
 

Product Name	Cat. #	Price	View
IntactProtein™ Cell-Tissue Lysis Kit (50 ml)	415M	$129

Case Study

Publications

1. PPARγ Acetylation inAdipocytes Exacerbates BAT Whitening and Worsens Age-AssociatedMetabolic Dysfunction. 
Publication: Cell        Product: IntactProtein™ Cell-Tissue Lysis Kit

2. Salidroside reducesneuropathology in Alzheimer's disease models by targeting NRF2/SIRT3pathway. 
Publication: Cell Biosci         Product: IntactProtein™ Cell-Tissue Lysis Kit

3. Ventromedial hypothalamic OGT drives adipose tissue lipolysis and curbs obesity.  
Publication: Advanced Science        Product: IntactProtein™ Cell-Tissue Lysis Kit

4. O-GlcNAcylation is a gatekeeper of porcine myogenesis.  
Publication: J Anim Sci        Product: IntactProtein™ Cell-Tissue Lysis Kit

5. PPARγAcetylation Orchestrates Adipose Plasticity and Metabolic Rhythms. 
Publication: AdvSci (Weinh)        Product: IntactProtein™ Cell-Tissue Lysis Kit

Customer Testimonials

"I was always having trouble dealing with extraction of large-sized proteins such as mTOR (MW 289) from cultured cells. My problem was that without sonication, the extraction efficiency of large proteins was low; with sonication, the large-sized proteins were fragmented into smaller-sized peptides. This dilemma was finally solved by using IntactProtein™ Cell-Tissue Lysis Kit. With this kit, I obtained excellent results without losing phosphorylation signals. Moreover, this kit was easy to use by simply mixing reagent A and B and lysing the cells for 15 min on ice."

"We have tested your IntactProtein™ Cell-Tissue Lysis Kit in liver, adipose tissue, and cultured cells. It worked really well! Extracting proteins from fat is always a headache. Your buffer gave us high protein yields and sharp protein bands. We’d like to switch to your buffer"